Verification and characterization of circRNAs in two holophilic archaea

Circular RNA (circRNA) is a special type of RNA, which forms covalent-closed continuous loop in most cases through 5’ to 3’ ends via back-splicing. CircRNAs demonstrate a certain degree of conservation and show some tissue specificity in multicellular animals. Archaea is one of the three domains of life, and archaeal organisms often live in high temperature, high salt, low oxygen, or other extreme environments.