Identification of the related genes at diapause termination in Helicoverpa armigera

By means of mRNA differential display PCR(DD-PCR), 56 cDNA fragments have been identified from the Helicoverpa armigeras brain that are expressed differentially between the diapausing pupae and the pupae of diapause termination by injecting 20-hydroxyecdysone. RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12(FKBP12)and two unknown genes were higher in the diapausing pupae, whereas the ecdysteroid-regulated 16 kDa protein(esr16), NADH dehydrogenase 1 alpha subcomplex subunit 6, and two unknown genes were higher in the pupae of diapause termination. These results provide new clues to researches on the molecular mechanism of diapause.